ING2 PHD domain links histone H3 lysine 4 methylation to.
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It was previously shown that PHD finger is a zinc-dependent fold and that zinc removal from the purified domain by addition of the excess EDTA results in the loss of the domain structure (Pascual et al., 2000).To ascertain that the SHPRH PHD domain is our samples is bound to zinc we have additionally recorded 1 H-15 N HSQC spectrum of 0.2 mM PHD domain in the presence of 10 mM EDTA (Figure 2.
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Neural Networks are a powerful machine learning model that can reach outstanding performance in many problems, such as classification and regression. In this PhD project, we want to investigate novel solutions for the generalisation problem affecting deep networks. Transfer learning and domain.
Our studies reveal that the PHD domain from KAP-1 corepressor binds zinc in a cross-brace topology between anti-parallel ss-strands reminiscent of RING (really interesting new gene) domains. Using a mutational analysis, we define the structural features required for transcriptional repression by KAP-1 and explain naturally occurring, disease-causing mutations in PHD domains of other proteins.